FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219196
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG73930.2296536153747706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG16600.059.7340055
GTCTACG16700.059.3763124
TACGGCC16900.058.672727
CTACGGC17400.057.1854866
ACGGCCA18900.052.28188
GAACGCG19300.051.1088124
CGGCCAT20050.049.2830929
CCCGATC20650.047.54455631
TGAACGC20950.047.24007423
CGGTATA3900.046.9712871
ATCTCGT20450.046.91039335
GCCCGAT21350.046.1296430
CGCCCGA21400.045.8432229
CTGAACG21900.045.65256522
TCTCGTC21600.045.08026536
GCCATAC21950.045.0185311
CGGTATG4000.044.932911
TCGTCTG21700.044.28433238
CCTGGTT35000.044.15070370
ACGCGCC22500.044.01796326
Produced by FastQC (version 0.11.5)