FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227053
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG40840.1265550953145176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA13450.034.2237532
TGGCCGT10400.033.7215670
GATTTCG5050.032.86108828
TCGCCGG12900.032.44708648
CGTTGGA6750.031.7980933
AGGACCG2850.031.22268970
CCGTCGC13800.030.53057545
TTCGTTG7300.030.33616631
GCGGCAC15100.030.2628733
CGCGCTA703.8534065E-529.5711362
ACCCGCG16050.028.6231425
CCCGTCG14700.028.4031444
CGGCACC16650.027.45971134
ATTTCGT6250.027.11250929
CGCCCTA5450.026.50655461
TCGTTGG8400.026.36931832
TTACTCG2300.025.95516457
AGTCCGC17700.025.9162357
AACGGGA5850.025.5290340
CAGTCCG17750.025.454616
Produced by FastQC (version 0.11.5)