FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3321194
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG43040.1295919479560664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA12450.034.1955632
TTTCGCG851.1185148E-732.76141745
CGTTGGA7950.031.3576833
GATTTCG6450.031.08827428
TGGCCGT11750.030.4773170
TCGCCGG13500.029.71812848
ATTTCGT6700.029.4220429
TTCGTTG8700.029.03175731
GCGGCAC14800.029.00938433
CCGTCGC13850.028.90278445
CCCGTCG14650.028.02023544
ATGGCCG9900.027.67950669
CGCCCTA5100.026.95493561
CGGCACC16500.026.2387234
ACCCGCG16750.025.77919425
TCGTTGG9950.025.7420232
CCCCCGA17700.024.55994819
AGTCCGC17950.024.3950127
CAGTCCG17550.023.9687026
CCGATCG6450.023.67793870
Produced by FastQC (version 0.11.5)