FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3321194
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG73460.22118551340270998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG15000.059.9304124
TACGGCC15250.058.286197
TCTACGG16100.055.6218535
CTACGGC16700.054.0383956
ACGGCCA18000.049.381368
GAACGCG18250.048.95640624
CGGCCAT18750.047.5898449
GCGTACT3250.046.784611
CGGTACT4350.046.075751
GCGTACC2800.045.6643561
TGAACGC19750.045.4060123
CGGTACG1400.044.4301871
GCGTATT4500.043.7719651
CTGAACG20650.043.5855322
CGGTATT5550.043.585171
CGGTATA3900.043.4175341
GCCATAC20750.043.16890711
CGCCCGA20850.042.91241529
GCCCGAT20750.042.79725330
CCCGATC20750.042.64297531
Produced by FastQC (version 0.11.5)