FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298727
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG73260.22208567123014425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG15800.059.5443424
TACGGCC16250.057.6851047
TCTACGG16550.056.6394545
CTACGGC16700.055.9243556
ACGGCCA18700.049.9431428
CGGTATA3700.049.5585981
GAACGCG19650.046.89250624
CGGCCAT21000.044.8007169
CGGTGTA4100.044.7236141
TGGCGCG18650.044.7168815
TGAACGC20700.044.5057923
GCCATAC20950.044.2496511
GCGTATA3550.043.8561
ATCTCGT20850.043.52808835
GCGCGGT19250.043.3237659
CCCGATC21000.043.31672731
CGGTATT6400.042.7063031
GCCCGAT21400.042.49218430
CGCCCGA21650.042.14646529
CTGAACG22100.042.1439222
Produced by FastQC (version 0.11.5)