FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5204688
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN179060.3440359921670617No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC153300.29454215122981436No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA148010.28437823746591534No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC107360.20627557309871408No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC104440.20066524640862238No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT69250.13305312441398984No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT66930.12859560457802657No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG54880.10544340025761391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT75450.050.905449
CCGAACT12400.047.3905167
TGGCGCG82250.046.99096715
CGCGGTG85300.045.35095610
GCCGGTT13200.042.8736843
CGGTTCA13750.041.9014245
ACTCTTC49050.041.8803185
TGTAGTC92450.041.84360526
GTAGTCC92450.041.7190927
TCTGGTC48400.041.5928910
TAGTCCC92800.041.4819928
CTACTCG92800.041.41438737
TACTCGG92500.041.11838538
CGTGCCT94350.041.0270820
TCTACGG6950.040.4623645
GGACTCT53100.040.1123283
CCTGTAG96700.040.11142724
GGGGACT53000.039.4860271
ACTCGGG99150.039.36853439
CCGGTTC14750.039.293154