FastQCFastQC Report
Thu 9 May 2024
EGAF00005318994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5401920
Total Bases402.7 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG446680.8268911794324981No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG207220.38360434808364435No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN184680.3418784432201884No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC157530.29161853563177537No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCAC135850.25148465730703157No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA132770.24578298086606246No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGT130030.2407107102659795No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG121670.22523473135477753No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC120110.2223468692612997No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT88210.16329379183697648No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG81810.15144615247911852No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT76700.14198655292932882No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC75980.1406536935015698No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA74280.13750666429713881No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC73990.13696981813873585No Hit
TGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA71230.13186052366565962No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAG70980.13139772525324328No Hit
CGCTTCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA68850.1274546827794562No Hit
GCCGGGAGGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGG64950.12023502754576151No Hit
GCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGC64250.1189391919909958No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGC63560.11766186837272674No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT61750.11431120786683252No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT61210.11331156329601327No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG61120.11314495586754339No Hit
GGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCG59640.11040518926603875No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG56380.10437029796812984No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG55090.10198225816006161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)