FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5401920
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN184200.34098987026834904No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC163250.3022073633078609No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA160220.29659824654937506No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC115100.21307238907647652No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC111410.20624148450921154No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT76410.1414497067709259No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT69130.12797301700136246No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG64370.11916133522895563No Hit
GGGGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTC55460.10266719981043776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT78700.051.778159
CCGAACT13000.047.07459367
TGGCGCG86500.046.82088515
CGCGGTG88550.046.1348310
GCCGGTT12900.045.4967233
CGGTTCA13500.043.4750485
TACTCGG97300.042.22511738
CTACTCG97650.042.15042537
CGTGCCT96150.041.81220620
GTAGTCC97000.041.70042427
ACTCTTC48600.041.6671185
TAGTCCC97750.041.4044528
TCGCGTA602.3561188E-741.35340560
TGTAGTC99500.041.11409426
TCTACGG6650.040.7739875
GGGGACT54750.040.249781
CCTGTAG100850.040.2131224
ACTCGGG104500.040.1416839
CTTAGGC14900.040.1218218
TCTGGTC49350.039.9991910