FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5340155
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG437860.8199387470962922No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG205210.384277235398598No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN178530.3343161387637625No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC152300.285197714298555No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA131030.24536740974746987No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC130410.24420639475820458No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG119400.22358901567463865No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG116750.21862661289794022No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC111730.20922613669453416No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT87760.1643397991256808No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG79960.14973348151879487No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT75910.14214943199214256No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC74890.14023937507431902No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC73530.13769263251722094No Hit
TGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA71810.13447175222442045No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA70990.13293621627087604No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAG69210.1296029796887918No Hit
CGCTTCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA65550.1227492460424838No Hit
GCCGGGAGGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGG64870.12147587476393476No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGC62520.11707525343365502No Hit
GCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGC62250.1165696501318782No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT61850.11582060820331994No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT60490.11327386564622188No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG59470.11136380872839834No Hit
GGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCG53830.10080231753572695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA130900.038.75992632
GCGGCAC134600.037.82176633
CGGCACC136200.037.19331434
CGCTTCC16950.036.5117451
GCACCCC142200.035.7876836
CCCGTCG133900.035.7606844
CCGTCGC133650.035.72902745
TCGCCGG134600.035.6434648
GGCACCC147300.034.54655535
CACCCCC147400.034.52818737
CCCCCGA143400.034.15549519
TTCCTCG20050.033.378424
ACCCGCG152700.033.34618425
GTGCCGT9100.031.7371311
CCCCGAC157100.031.10553220
GCAGTCC24600.030.6925625
CCCCCGT159300.030.0554442
TAGTCCG7600.029.805216
GGCAGTC24700.029.734624
CCGCGCG177000.028.90072327
Produced by FastQC (version 0.11.5)