FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5340155
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN177930.3331925758709251No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC160680.30089014270185044No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA159650.2989613597358129No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC113390.2123346606980509No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC110340.20662321599279423No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT75260.1409322388582354No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT69140.1294718973512941No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG63840.11954709179789724No Hit
GGGGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTC55470.10387338944281581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT76300.049.696869
CCGAACT13250.048.3750267
TGGCGCG80500.046.93214815
CGCGGTG85250.044.43916710
GCCGGTT14300.044.1530533
CGGTTCA14000.043.6302075
ACTCTTC47650.043.570025
TCTGGTC47750.042.6878510
CTACTCG90350.042.21578237
GGACTCT54500.041.9960333
TACTCGG90900.041.94847538
GTAGTCC91050.041.6954227
TACGGCC6050.041.406347
TAGTCCC91850.041.39996328
CGTGCCT92700.040.91145320
TGTAGTC93700.040.63549426
GGGACTC54300.040.2548832
CGAACTT16450.040.01732368
GGGGACT56350.039.991371
TCTACGG6200.039.850715