FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3818723
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA220100.5763706872690164No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC195200.5111656435934211No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG178550.4675646806537159No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC175080.45847787336237794No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT93220.24411301893329262No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG49370.12928405647647132No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA45150.118233241845507No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC44410.116295421270409No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC40090.10498273899416115No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA38260.1001905610854728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG35450.064.0473564
TCTACGG36000.063.1648525
TACGGCC36650.062.136457
CTACGGC37350.061.0644346
CCGAACT15050.057.75418567
GCGTCTA8000.057.0405962
ACGGCCA40450.056.2984248
GCCGGTT15600.055.381863
CGGCCAT41100.055.3239829
CGAACTT16400.054.52871768
CGGTTCA15700.054.368765
GCCATAC42400.053.8715211
GGGTCTA30250.053.8264432
CCATACC42500.053.8253712
GCGCGGT88300.053.6544959
CGTCTAC9550.053.5562863
GGCCATA43500.052.66813310
CATACCA43450.052.6485213
CCTGGTT60150.052.29898570
CGCGGTG93050.050.95201510