FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3836544
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA215910.5627721199079171No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC194060.5058198211723884No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG180940.4716223768057919No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC178100.46421988122643715No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT90530.23596757915457248No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG50720.1322023154172088No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA43870.1143477046008074No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC43460.11327903446435125No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA38800.10113268608414239No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC38570.10053318820271578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG32400.063.7717325
GTCTACG33350.061.955154
TACGGCC33500.061.5721867
CTACGGC33900.060.9467966
ACGGCCA36100.057.329048
CGGCCAT37100.055.690669
GCCATAC37950.054.44259311
GCGCGGT89950.054.312949
GCCGGTT15050.053.9528963
CCGAACT15100.053.08876867
GCGTCTA7450.052.4096452
CCATACC39500.052.13129812
GGGTCTA28700.052.1308822
GGCCATA39850.051.8475110
CGCGGTG95750.051.167310
CATACCA40600.050.71821213
TGGCGCG96650.050.54586415
CCGGTTC16350.050.295754
TACCACC41650.049.52190815
GGGGACT49350.049.4757421
Produced by FastQC (version 0.11.5)