FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169032
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG184300.5815656011046906No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG86570.2731749000956759No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78440.24752037846257155No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG56730.17901365464280575No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG52620.16604439462902237No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC45870.1447445150443416No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC44760.14124186817930523No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC44420.14016898535578057No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT42190.1331321362485453No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC40600.1281148312797094No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC37480.11826955360501251No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA36570.11539801428322592No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT35260.11126425987493974No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA34250.10807716678152823No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG33510.10574206887150397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA57050.035.90720732
CGTTGCG1151.8189894E-1235.8145141
GCGGCAC58050.035.376933
CGGCACC60100.034.36250734
TCGCCGG58950.033.0867648
CCGTCGC58900.033.000845
CCCGTCG59550.032.8422244
TAGTCCG3500.032.3343546
AGGACCG8950.031.27925370
GCACCCC66850.031.19039236
GGCGATC11850.030.37424970
CCCCCGA68600.030.22867219
GGCACCC69700.029.79803335
ACCCGCG70050.029.42218825
CACCCCC71350.029.24518837
TATCGGA2900.028.3816998
CGGAATT2800.028.17052711
TTATCGG2800.028.1705277
GTGCCGT5000.027.4577921
ACCGGTT1503.8198777E-1127.4360773