FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3220194
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG180690.5611152619997428No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG85910.2667851688438647No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78850.24486102390104447No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG55730.17306410731775787No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG51660.160425117244489No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC47200.14657502001432213No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC44180.13719670305577863No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC43640.13551978545391985No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT41080.12756995385992273No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39530.12275657926199476No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT37110.11524150408329435No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC36310.11275718171017027No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA36100.11210504708722517No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA34160.10608056533239923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA57650.039.45078732
GCGGCAC59550.038.33701733
CGGCACC62650.036.35016634
CCCGTCG59750.035.4032344
TCGCCGG60150.035.24695648
CCGTCGC60300.035.2293245
GCACCCC66550.034.2050136
CACCCCC70450.032.5799137
CGCGACT851.2684723E-732.2951932
CCCCCGA70000.032.14179619
GGCACCC70800.032.13810335
ACCCGCG70150.032.0555725
TGCGACG1001.5041223E-830.9371871
CGTACGT2150.030.35582419
AGGACCG7750.030.22499770
AGTCGTA3300.030.15582310
TAGTCCG3650.030.0845996
CCCCGAC76900.029.18075420
CGTTGCG953.3106335E-728.9470771
AGTCCGC80950.028.0626227
Produced by FastQC (version 0.11.5)