FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3220194
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA93490.29032412332921553No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC82580.25644417696573557No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77800.24160035078631906No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC57250.17778431982669368No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT40730.12648306282168092No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC39950.12406084850788493No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG38040.11812952884205113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT6450.048.59644767
GCGCGGT36450.047.2470479
TGGCGCG38200.044.72266415
GTACGCA4950.044.0722165
TCTACGG4400.042.1033175
GGGGACT32250.041.44571
GTCTACG4550.040.713394
AGTACGC5950.040.20431564
CGCGGTG43450.039.6353210
TCTGGTC25050.039.4414810
TACGGCC4800.039.309427
GCCGGTT7750.038.9525453
ACTCTTC25950.038.867375
CGGTTCA7650.038.5666775
GTTGATC8400.038.389910
CTACTCG46250.037.7805637
TACTCGG46550.037.334238
GCGGTAT1400.036.9097141
TGTAGTC47100.036.70723326
GGACTCT31500.036.591793