FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073614
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG105580.3435044218304576No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG45570.14826194831231249No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC42950.13973778099657277No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG41240.13417429774851364No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC33420.10873193576031344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT1151.8189894E-1236.03178419
CGCGGCA31200.035.73208232
TCGCCGG31000.034.28909748
TGGCCGT15650.033.37967370
GCGGCAC33400.033.28296733
CCGTCGC32400.033.0774145
CCCGTCG32400.032.9521744
CGGCACC35350.031.55371534
GTTCCGT3450.030.0460661
ACCCGCG37950.029.78408625
CGCCCTA8600.029.0958861
CCCCCGA39100.028.9667319
GCACCCC39600.028.26814336
ATGGCCG15450.027.46093269
CACCCCC41700.026.76933737
CCCCGAC42150.026.63011720
CGTCGCC40350.026.39996746
GTCCGTT2800.025.8932062
GGCACCC43300.025.84712435
AGTCCGC44000.025.5701897
Produced by FastQC (version 0.11.5)