FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3146092
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG106760.3393416340018029No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG46360.14735741993558993No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC42700.13572393941435915No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG42570.1353107283575941No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC33200.10552774680460712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT17000.042.93943870
CGCGGCA33300.034.741232
GCGGCAC35050.033.1165333
ATGGCCG17000.033.1144369
TCGCCGG33750.032.31920248
CCCGTCG34400.031.53837644
CGGCACC36800.031.36089534
TACGGGT8350.031.0271056
CCGTCGC34900.031.00278945
GTTCCGT3700.030.8562011
TCGCCGT2700.030.7523081
GTACGGG8350.030.6143865
CCCCCGA40350.029.0392419
ACCCGCG40400.029.0277925
TATCGGA2200.028.2628738
GTCGGTT2100.027.9677984
GCACCCC42600.027.51412236
CCCCGAC43800.026.5193820
CACCCCC44350.026.35825537
GGGTACG9700.026.3565543
Produced by FastQC (version 0.11.5)