FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3146092
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG157680.5011932263900737No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC116590.37058674698641997No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC100000.3178546590500214No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA69860.22205326481234497No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT53790.17097402110300652No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG43290.13759928190275428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG33200.062.1949654
TACGGCC33450.061.3250277
TCTACGG33900.060.8088465
CTACGGC34700.059.4097486
GCGCGGT54050.054.692219
ACGGCCA37800.054.541868
GCCATAC38350.053.75964411
CGGCCAT38950.052.754199
GGGTCTA29950.052.228472
CCGAACT8900.051.52824867
GCGTCTA7100.051.0665972
TGGCGCG58300.051.05331415
CCTGGTT58900.051.037370
CGCGGTG59050.050.47057710
CCATACC40850.050.46957812
CATACCA41200.050.1238513
GGCCATA42050.048.94717810
CTGAACG42600.048.3621722
CGGTTCA9300.047.5246735
GAACGCG43650.047.04942724
Produced by FastQC (version 0.11.5)