FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4164255
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG139250.3343935469849949No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG50530.1213422328843935No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA42960.10316371115601711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA43750.034.27796632
CCCGTCG42750.033.60241744
TCGCCGG42600.033.35068548
CCGTCGC44400.032.20321345
GCGGCAC46700.032.04294633
CGGCACC48700.030.87294234
ACCCGCG49850.030.20275925
GTTCCGT4250.029.4596981
CCCCCGA52550.029.09452819
AGTCCGC58000.026.7739227
CCCCGAC56700.026.72398420
CACCCCC57000.026.63261837
GCACCCC57000.026.56719636
CAGTCCG56550.026.3571116
CGTCGCC55050.025.91934446
TCCGCCC63400.025.1495679
GTCCGCC62600.024.9172528
CCGCGCG61500.024.8780827
CCCGCGC61600.024.61181826
GTGCCGT6650.024.0575221
Produced by FastQC (version 0.11.5)