FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4164255
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC79440.19076641560135005No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC71510.1717233935001579No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG46120.11075210331740011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG13250.052.56854
TCTACGG13350.052.1747325
TACGGCC13600.051.216877
GCGCGGT42250.047.7366759
CTACGGC15350.045.1509676
CGGGTAG6300.043.111371
ATCTCGT15600.042.50885835
TGGCGCG47450.042.5021915
GAACGCG15950.041.5210824
CGCGGTG50000.040.82264310
CCGAACT6950.040.02594867
CCCGATC16700.039.47768831
GCCATAC17600.038.97754311
GCCCGAT17050.038.66217830
TGAACGC17250.038.58878723
CGTCTGA17400.038.5387639
CGGCAAA8400.037.7224461
TCTCGGA17850.037.42349646
CTACTCG53700.037.3180237
GCCGGTT7900.037.2834743