FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4153405
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG140790.33897488927759273No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG52040.12529478825204862No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA42070.10129038704388327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA41550.036.02256432
TCGCCGG40150.035.2952248
CCCGTCG41600.034.53708344
GCGGCAC44450.033.91372333
CCGTCGC43500.032.7961845
CGGCACC47350.031.83894534
ACCCGCG47550.031.5151825
CCCCCGA50750.029.78347219
CCCCGAC55050.027.64698620
CGTCGCC51600.027.45128646
GTAGCGT3050.027.27423710
GCACCCC55800.027.27140436
CGTAGCG2800.027.2326689
CACCCCC55350.027.18354237
AGTCCGC57650.026.3931227
CAGTCCG56500.025.8874666
CCGCGCG58800.025.78916527
GTTCCGT3400.025.7039721
GTGCCGT6700.025.5658631
CCCGCGC59250.025.52888326
Produced by FastQC (version 0.11.5)