FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4158302
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC77930.1874082257613805No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC73830.1775484320282654No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG47580.11442170385893087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG11750.053.356644
TACGGCC11700.052.6965147
TCTACGG12550.049.4034235
GCGCGGT44650.048.252159
CTACGGC13700.045.5054176
TGGCGCG48200.044.76693715
GAACGCG14300.043.3930524
CGGCATA3950.041.491921
CGCGGTG53350.040.5782510
GCCCGAT15150.040.5379230
ACGGCCA15350.040.166078
ATCTCGT15700.039.3710835
CCCGATC15800.038.87631231
TGAACGC15900.038.80422623
CTACTCG56200.038.26345437
TACTCGG56500.037.94326838
CGTGCCT56900.037.81672320
CGTCTGA16800.037.23401339
CCGAACT7100.037.22500267
CGGGTAG6150.036.8553921