FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4163719
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC75200.1806077691602147No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC67950.16319545098984825No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG45780.10994978287439665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG12000.050.8217624
TACGGCC12100.049.5491757
TCTACGG12850.048.0062525
GCGCGGT45800.046.536169
CTACGGC13450.044.8340386
TGGCGCG48300.043.69169615
CCGAACT7500.041.76848267
TGAACGC14500.040.64801423
CGCGGTG53350.040.2102810
GAACGCG14750.039.25768324
CGAACTT8300.038.9192668
ATCTCGT15400.038.78103635
CCCGATC15550.037.94024731
ACGGCCA16100.037.8845838
GCCGGTT8250.037.801763
CTACTCG56550.037.5899537
TACTCGG56550.037.47252738
CGTGCCT56350.037.3984220
GCCCGAT15700.037.13164530
GCCATAC16600.036.5351511
Produced by FastQC (version 0.11.5)