FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005319038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3998640
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG385540.9641778204589561No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC186720.4669587659804334No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC185600.4641578136566433No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG143030.3576966168497289No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG132770.3320378928835804No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA117040.29269951783606424No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG100070.25026008843006625No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT93660.2342296380769462No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA88580.22152531860832686No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT81230.20314406898345438No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT80810.2020937118620331No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG73950.1849378788788188No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG68720.17185843186683472No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG65100.1628053538202989No Hit
GCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGC65080.16275533681451695No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC63840.15965428245603505No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG61840.15465258187783848No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT60970.15247684212632295No Hit
GGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCG59840.14965088129964188No Hit
TGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA59180.148000320108837No Hit
GCCGGGAGGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGG56060.14019766720685034No Hit
CGCTTCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA55470.13872216553628233No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG54610.1365714342876578No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG52930.13237000580197267No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA49670.12421723385951224No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC49660.12419222535662124No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC48930.1223666046455795No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT48080.12024088189984594No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG47920.11984074585359022No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC47600.11904047376107878No Hit
GCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACC47000.1175399635876198No Hit
GTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCG45770.1144639177320289No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAG43850.10966228517696017No Hit
TGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCC43550.10891203009023068No Hit
GTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGG43110.10781165596302743No Hit
GGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAG42380.10598603525198567No Hit
ACTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG42270.10571094172018487No Hit
GGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAA42180.10548586519416603No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42030.10511073765080128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)