FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3998640
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA193240.48326430986535424No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC146120.36542424424304265No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC124700.3118560310505572No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG71880.17976111878038534No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT67450.1686823519996799No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC56750.14192325390632815No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT55120.13784686793509796No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA53830.13462077106216114No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG52900.13229498029329972No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA43120.10783666446591841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG15250.058.797385
TACGGCC15700.057.5538447
GTCTACG15600.057.4782074
GCGCGGT65150.053.8836489
CTACGGC16850.053.4197046
CGGCATA2200.050.5920371
CCGAACT10650.050.36202667
CGGTTCA10700.049.503475
CGCGGTG71250.049.46481710
TGGCGCG71250.049.36516615
GCCATAC18550.047.95820611
ACTCTTC51950.047.2485775
GCCGGTT11300.047.179573
ACGGCCA19200.046.881948
GGGGACT49050.046.8015061
CGAACTT11850.045.6497668
CGGCCAT20050.045.412449
GGACTCT54450.044.6324733
TCTGGTC53400.044.5398710
GTAGTCC79900.044.2750927