FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3982973
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA188610.4735407445644246No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC151050.3792393270052295No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC125810.3158695778254083No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG72020.18081970427617763No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT67260.1688688324023286No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC56130.14092488199141695No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT55740.13994571391771926No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA55210.13861504961243776No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG52370.13148469748602362No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA40270.10110538032770998No Hit
GGGGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTC40110.10070367034875707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG15050.055.666575
GTCTACG15300.054.9832534
TACGGCC15900.052.6900187
GCGCGGT69150.052.0155839
CCGAACT9100.050.4213567
CTACGGC16800.050.0715146
TGGCGCG74600.047.9352815
CGCGGTG75600.047.62353510
GGGGACT44350.047.6002961
GCCATAC18250.046.85373311
ACTCTTC45700.046.3607865
TCTGGTC46350.045.8594410
CGGTTCA10150.045.0218625
CGGCCAT19000.044.822049
CCATACC19000.044.45763412
ATCTCGT18750.043.73048835
CATACCA19700.043.4051113
GCCGGTT10450.043.3980873
GGACTCT48850.043.158693
CGAACTT10600.042.91849568
Produced by FastQC (version 0.11.5)