FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3984486
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA182410.45780057954777603No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC142420.35743631675453247No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC122710.30796945954886024No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG69550.17455199993173526No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT67230.16872941704400518No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC54210.13605268032062354No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT53650.13464722927875766No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA52550.13188652187509253No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG50050.12561218686676273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC16150.056.1609887
GTCTACG16750.054.137684
TCTACGG16700.054.1040765
GCGCGGT66550.052.5386929
GCCGGTT11050.050.74183
GCCATAC17600.050.5506111
CTACGGC17950.050.529246
CGAACTT11850.049.58648768
TGGCGCG72250.048.7715715
CCGAACT11800.048.16267467
CGGTTCA11700.047.9330525
CGCGGTG73850.047.9078110
CGGCCAT19600.046.8053789
CCATACC19500.045.44420612
CATACCA19500.045.44420613
GGGGACT47300.045.4430121
GCCGAAC12550.045.13979766
ACGGCCA20400.044.969878
ACTCTTC49450.044.8743025
CTTAGGC12850.044.45656218