FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3583112
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG203820.5688351354911596No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC93890.2620347898698115No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC87340.2437545909812476No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65290.1822159061731813No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG58380.16293099406326123No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT54080.15093025280817346No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA50020.13959932036732317No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG40260.11236042858833327No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC40130.11199761548062132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG40350.062.6861344
TCTACGG40800.061.9947435
TACGGCC41850.060.4393237
CTACGGC42600.059.7786146
ACGGCCA44600.057.02098
GGGTCTA32200.056.3606572
GCGTCTA8200.056.167992
GCCATAC45600.055.3905211
CGGCCAT45900.055.1813139
CCATACC47450.053.15850412
CATACCA47700.052.80785413
GGCCATA48450.052.34430310
CCTGGTT69950.052.03435570
CCGAACT10450.051.01922667
CGTCTAC10250.050.9639433
GCGCGGT49750.048.9077159
GGTCTAC41600.048.659233
CGGTCTA5300.048.638852
TACCACC52600.047.30182315
TGGCGCG51250.047.13966815