FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3664554
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG205580.5609959629466506No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC93950.256374991335917No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC86810.23689103776339493No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65350.17833002324430203No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG57410.1566629936412453No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT53520.1460477864427704No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA51350.14012619270994506No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC41140.11226468487024616No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG40870.1115278967099407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG42800.063.593774
TCTACGG43350.062.8679245
TACGGCC44750.060.8326237
CTACGGC44900.060.6235966
ACGGCCA48000.056.856968
GCCATAC49300.055.21825411
GGGTCTA36150.055.046182
CCTGGTT72350.053.8856970
CGGCCAT50500.053.770019
CATACCA51350.052.94471713
GGCCATA51600.052.890210
CCATACC52450.051.9661112
GCGCGGT49550.050.708199
GCGTCTA9650.050.216762
GGTCTAC44900.048.75583
TACCACC55750.048.69781515
CTGAACG56650.048.06431222
TGGCGCG52600.047.95540215
GAACGCG56550.047.75066824
TGAACGC56900.047.62418423