FastQCFastQC Report
Wed 8 May 2024
EGAF00005319052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631690
Total Bases271.2 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG147860.40713827446725903No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG72000.1982548069906848No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCAC69090.19024200854147794No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67340.1854233153160099No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC52220.1437898058479661No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGT42550.11716308385352275No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG41440.114106655579083No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG40620.111848753610578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)