FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605860
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG128450.35622569927839687No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC100920.2798777545439923No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC96520.26767539505138854No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG80950.22449568202869774No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG57510.15949038509537253No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT57310.15893573239116326No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT50650.14046579734099493No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT44790.12421447310766365No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG43440.12047056735425114No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT40610.11262223158969012No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA40530.11240037050800641No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG39920.11070867976016817No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG38560.10693704137154521No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG38220.10599413177438946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT26400.050.7432370
ATGGCCG29100.035.6573369
GTTCCGT3700.034.582221
CGCGGCA37000.034.02995732
AGGACCG6650.033.316270
TGCGACG1056.2755134E-1032.935451
GCGGCAC39350.031.74545533
GTGCCGT4700.031.6390531
CGGCACC40450.030.97461934
CCGTCGC39650.029.7502745
CCCGTCG40400.029.60953544
TCGCCGG40100.029.55993348
GTCCGTT3300.029.2435262
TATGGCC34650.028.04109668
GCACCCC46100.027.71976736
GTATGGC34700.027.53266567
CGCCCTA10350.027.31643761
CCCCCGA46450.027.09140219
TACGGGT15200.026.7539526
CTTACCC19200.026.56700970