FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605860
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG279600.7754044804845446No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA152340.42247896479619285No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC133660.37067440222304804No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC116230.3223364190512111No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG81010.2246620778399605No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT76080.21098988868120225No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG53810.14922931006750123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG57100.064.021954
TCTACGG57400.063.5072635
TACGGCC58150.062.390187
CTACGGC59300.061.414366
ACGGCCA62150.058.5410278
CCTGGTT91700.058.29303470
GGGTCTA47350.058.086772
GCCATAC63550.056.93398711
CGGCCAT63800.056.8133969
TCGCGTA252.2971926E-456.45890860
GCGTCTA9650.055.360332
GCCGGTT12050.054.6205673
GGCCATA66900.054.2337910
CCATACC67250.053.852812
CATACCA67300.053.76158513
GCGCGGT61500.053.5037089
CCGAACT13100.052.7385367
CGGTCTA7000.052.684162
TACCACC69650.052.19503415
CGTCTAC12400.051.9672623