FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3600213
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG280190.7782595085346339No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA154870.43016899277903836No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC142030.39450443626529874No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC120610.3350079564736864No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG80290.22301458274829852No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT77660.2157094594125403No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG54970.1526854105576531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG53900.063.320114
TCTACGG53900.063.128235
TACGGCC54650.062.324967
CTACGGC55300.061.592396
ACGGCCA58050.058.912138
GGGTCTA42000.057.4635282
CCTGGTT86100.057.202470
CGGCCAT60200.056.7508549
GCCATAC60900.055.9217311
GCGTCTA10250.055.837732
CCATACC62100.054.89661812
CGGTCTA7800.054.3693432
CATACCA62850.054.18668713
GGCCATA63300.053.96483610
GCCGGTT11650.053.5616463
CGTCTAC12650.052.3253333
GCGCGGT66500.052.1520739
TACCACC65700.051.73478315
CCGAACT12150.050.8988767
ATACCAC67650.050.54576514