FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3699562
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG137330.371206104938909No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC103540.27987096850924514No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC97110.2624905326630558No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG85090.23000020002367846No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG61680.16672243903467493No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT59950.16204620979456488No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT54180.1464497689185909No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG45530.12306862271804068No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT44550.12041966048953903No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG41520.11222950176264108No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT41250.11149968563846206No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA41150.11122938337024761No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG40600.10974272089506812No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG40040.10822902819306718No Hit
GGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACT37590.10160662262181308No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG37180.10049838332213382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT27350.053.36413270
ACGATCG551.474582E-743.8953976
ATGGCCG30300.037.72434669
CGCGGCA43850.037.23541332
GCGGCAC45950.035.62056733
AGGACCG8050.035.11197770
CGGCACC46750.034.94743334
GTCCGTT4050.034.0689772
GTTCCGT3600.033.5784381
CGTACTA1450.033.3053632
TCGCCGG44550.033.170748
CCGTCGC44700.032.99489645
CCCGTCG45400.032.92701344
GCACCCC52750.031.19251636
CTTACCC18800.030.8893770
CCCCCGA53350.030.59133119
TATGGCC35350.030.11231268
CACCCCC55250.029.79242337
ACCCGCG55200.029.72689425
GTATGGC35300.029.65719267