FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3699562
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG281740.7615496104674013No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA159710.4316997525653037No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC141000.3811261981823794No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC123100.33274209217199224No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG81810.2211342856262444No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT77550.2096194090003087No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG56340.1522882979120231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG57500.064.704954
TACGGCC57700.064.1307757
TCTACGG58250.063.8126375
CTACGGC59200.062.616426
ACGGCCA61450.060.217198
GGGTCTA45300.059.295692
CCTGGTT90950.059.1069570
CGGCCAT63000.058.4619529
GCCATAC63200.058.11246511
CCATACC64850.056.6870712
GGCCATA65850.056.08882510
GCGTCTA12050.055.227342
CGGTCTA7750.054.725192
CCGAACT10950.054.4918967
CATACCA67750.054.3616813
GCGCGGT69550.053.947889
GCCGGTT11250.053.3303873
TACCACC69900.052.53378315
CTGAACG71300.051.98179622
CGTCTAC13800.051.2215583