FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3659130
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG134490.367546383976519No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC101360.27700573633623293No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC95640.26137360520123637No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG82240.2247528784164542No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT60020.16402806131512135No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG58430.1596827661220017No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT52260.14282083446065047No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT44810.12246080352433503No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG44640.12199621221437884No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA41950.11464473795683673No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG40690.11120129648304379No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT40370.11032677166430273No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG39860.10893299773443416No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG38850.10617277877528264No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG37240.10177282578099164No Hit
GGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACT36900.10084364316107929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT25950.051.9747370
CGCGGCA45250.037.31958832
ATGGCCG28950.036.81429369
AGGACCG7950.036.62970470
GCGGCAC46400.036.10920733
CGGCACC47650.035.0248334
CCGTCGC47950.032.43683245
TCGCCGG48150.032.4240148
CCCGTCG48850.031.89373444
CCCCCGA54850.030.95819719
CTTACCC17750.030.50936770
GCACCCC55050.030.46296336
GTTCCGT4200.030.4416941
TATGGCC34500.029.36917368
GTATGGC34350.029.31651367
GGCACCC57150.029.15289735
ACCCGCG57500.029.14396725
CACCCCC57550.028.9729537
GACGTAT4450.028.91569144
CCCCCGT56000.027.91999842