FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3659130
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG276710.7562180081057519No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA157860.43141402464520257No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC140820.38484557804724073No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC116480.31832703402174833No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG80560.2201616231180636No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT76780.20983129869668474No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG53660.14664688054264266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG55700.064.140224
TACGGCC56200.063.5695767
TCTACGG56250.063.513075
CTACGGC57600.061.9047476
ACGGCCA59750.059.7349248
CGGCCAT61050.058.3491639
CCTGGTT88550.057.9027270
GCGTCTA11150.057.525972
GGGTCTA45500.056.9944152
GCCATAC62150.056.9280311
CCATACC63700.055.65108512
CATACCA64450.055.16474513
GGCCATA64950.055.16407410
GCGCGGT66400.053.9594359
TACCACC68050.052.39843415
CCGAACT12300.051.9447867
CTGAACG69450.051.50486822
CGTCTAC13500.051.343823
GCCGGTT12600.051.1796073
CGCGGTG70650.050.7622810