FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716123
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA3700.053.5448041
GCGTACT3050.050.1414681
CGGTACA5750.048.357781
CGGTATT6150.048.038341
CCGTATA2100.047.997981
CGGTATG4200.047.997981
CGGTGTA4150.047.738741
CGGTACT4750.047.5624241
TACGGCC4450.047.4673967
GCGTATT5600.046.549761
GTCTACG4550.046.4241564
CTACGGC4550.046.4241566
GCGTACA4200.046.3428731
CGGTAAT4200.045.5153241
TCTACGG4800.044.7276465
GCGTATA4200.044.687771
CGGTATC3350.044.613661
GCGTGTT5150.043.868251
TGGCGCG13250.043.1214815
GCGTATG4750.042.4403151
Produced by FastQC (version 0.11.5)