FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741904
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG29700.10831889081455806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG400.002356676235.1897367
CGCGGCA9800.035.06380532
TCGTTGG9200.034.70962532
TTCGTTG9500.033.61230531
CGTTGGA9950.033.1434833
GATTTCG7200.032.77233528
CCGTCGC10050.031.84494645
AAACGGG9400.031.7887139
TCGCCGG10100.031.70528848
CCCGTCG10350.031.25114644
TCGCCGT901.8969513E-730.8468041
GCGGCAC11300.030.41265133
CGGGAAT6100.030.19891442
ATTTCGT8800.029.57720629
TTACTCG1900.029.39937457
GAAACGG10650.029.3588938
CGGCACC11950.029.34367234
CGAAACG953.0210504E-729.25106237
AACGGGA8200.029.24052640
ACCCGCG12600.028.08390825
Produced by FastQC (version 0.11.5)