FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830981
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG31770.112222582913838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG551.3787758E-744.27153445
GATTTCG6750.042.16662228
TCGTTGG8800.040.63908432
CGTTGGA9100.039.30211633
TTCGTTG9300.039.1939431
TTACTCG1850.035.8347957
ATTTCGT7950.035.3677529
CGCGGCA10450.034.886932
GCGGCAC11100.032.846333
TCGCCGG10650.031.70101448
GAAACGG10400.031.40835438
CGACGAT901.9053914E-730.83127221
CCCGTCG11400.030.8126344
AACGGGA7900.030.35904140
CCGTCGC11350.030.34090445
TACCGGT1151.5734258E-930.22887841
AAACGGG9700.030.09675839
CGGGAAT6850.029.94680842
GGAAACG10350.029.5403837
AGCGTTC1900.028.38774769
Produced by FastQC (version 0.11.5)