FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830981
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC2250.055.572751
CGGTACA5350.051.2879371
GCGTACT3700.050.691361
GTCTACG5150.050.4211164
CGGTATA4400.049.7312971
CGGTGTA4550.049.618531
TCTACGG5250.049.4607125
GCGTATT5750.048.9281851
CGGTATG4100.048.28731
CGGTATT6900.047.8207551
CGGCATA3900.046.3106271
CGGTAAA4650.045.563681
TGGCGCG14550.045.2107115
CGGTACT4400.044.9949841
CTACGGC5700.044.9524776
GCGTATA4900.044.6566731
GAACGCG5700.044.36617724
GCGTACA4300.043.6181451
CGCGTAA404.8090482E-543.416211
CGGTACC2800.043.416211
Produced by FastQC (version 0.11.5)