FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2805820
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATG3100.056.0404361
CGGTATA4650.054.546031
TACGGCC4800.051.9261937
GCGTACT3050.051.2632181
CTACGGC4950.050.3526736
GTCTACG4950.050.350884
TCTACGG5000.049.849155
CGGTACT4950.049.1344451
GCGTATT5850.048.108561
CGGTATC3350.047.7096521
CGGTATT5900.045.934161
CGGTAAT5100.045.645481
GCGTACA4600.045.3196561
CGGTAAA5450.045.2642171
CCGTACA2550.044.9642071
CGGTAAG3050.044.428121
GGGTATA18000.044.010421
CGGTGTA4300.043.6333431
GCGTGTA3800.042.9741631
CGGTACA5750.042.9026071
Produced by FastQC (version 0.11.5)