FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3292519
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG296240.8997366454073613No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA155520.4723435157094006No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC145950.4432776242141655No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC143080.43456089395383896No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG86000.2611981889853939No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC77350.23492651067465367No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT73900.22444821123279776No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG58930.17898150321987513No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC57990.17612654627049987No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC53770.1633096118807515No Hit
GGCCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC50600.15368172514722012No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG45660.13867801522178005No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40230.12218608305677202No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG35140.10672679489472953No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT33110.10056130275937662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG58800.064.1437154
TCTACGG59000.063.9846655
TACGGCC58750.063.6687137
CTACGGC60200.062.5947886
ACGGCCA60650.061.5605668
GGGTCTA45450.060.7176632
GCGTCTA9800.060.1154982
CGGCCAT61950.060.1028569
GCCATAC62150.059.8004211
CCTGGTT87800.058.89694270
CCATACC63000.058.83046712
GGCCATA63850.058.42402610
CATACCA63950.058.06424313
CGTCTAC12350.057.182573
CGGTCTA7250.056.5490572
CCGAACT11900.055.70363267
TACCACC67650.055.55043815
GCGCGGT74350.055.035929
CTGAACG69600.053.77235422
ACCACCC70500.053.50021716