FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3289666
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG301670.917023187156386No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA159670.48536842342049313No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC151420.4602898896118937No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC146890.44651949468426283No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG87460.2658628565939521No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC78160.23759250939153093No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT77400.23528224445885995No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC60760.1846996017224849No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG59890.1820549563390326No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC57180.17381703796069267No Hit
GGCCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC51290.1559124847324926No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG50720.15417978603298937No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39410.11979939604810945No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG37420.11375014971124729No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT36240.1101631594210476No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA34390.10453948820336167No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC33600.1021380286022958No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC33050.10046612634838917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG61350.064.11284
TCTACGG61450.064.008465
TACGGCC62050.063.3895267
CTACGGC62250.063.1858676
GGGTCTA44100.061.70892
ACGGCCA64600.060.9939428
CGGCCAT65900.059.68629
GCGTCTA12950.059.585092
GCCATAC65900.059.26627311
CCTGGTT90650.058.68843570
GGCCATA67800.057.96100610
CGTCTAC15000.057.6335953
CCATACC68200.057.41906412
GTCGTAC1200.057.4039733
CATACCA68650.056.99337813
GCGCGGT73600.055.4541139
TACCACC71550.054.4924815
CGGTCTA7450.054.098962
CGCGGTG76950.053.1278310
CCGAACT11600.052.86311767
Produced by FastQC (version 0.11.5)