FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338179
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG296700.8888079398977707No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA163090.48855978064687366No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC149030.44644100870564457No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC146400.438562461749355No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG87690.26268813026503374No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC76590.22943646820616867No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT75750.22692012621252483No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC59890.17940919285634474No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG57390.17192007978002377No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC54570.16347236022993375No Hit
GGCCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC50990.15274795030464214No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG50300.15068095509557755No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40840.12234215121477907No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA35600.10664497020681037No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT34980.10478767016388277No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG34070.10206163300410193No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC33760.10113298298263815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG58300.064.318944
TCTACGG58450.064.213755
TACGGCC59000.063.4993747
CTACGGC59250.063.1733366
ACGGCCA61350.061.17938
GCGTCTA11900.060.4754182
GGGTCTA44350.059.7052152
CGGCCAT63250.059.3388339
GCCATAC63200.058.89544311
CCTGGTT86450.057.92605670
GGCCATA64550.057.8770710
CCATACC64950.057.4137412
CGTCTAC14250.057.0264933
CATACCA65550.056.93988813
CCGAACT12400.055.76704467
CGGTCTA6700.055.5045432
CGGTTCA12800.054.0719645
GCCGGTT12800.053.8021433
TACCACC69750.053.5606115
GCGCGGT70400.052.9209569
Produced by FastQC (version 0.11.5)