FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4004638
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG138640.3461985827433091No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG64090.16003943427595702No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC46530.11619027737338557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA48100.034.72885532
TGGCCGT16250.033.86816870
AGGACCG6900.033.66102270
GCGGCAC50350.033.46314633
CGGCACC52550.032.06703634
CCGTCGC48900.030.30797245
CCCGTCG48650.030.30774144
TCGCCGG49100.030.22974648
GTTCCGT4550.029.7259391
CGTACGT2100.029.6412419
GCACCCC58200.029.0829936
CCCCCGA58400.029.01525519
ACCCGCG58750.028.56986425
CACCCCC60300.028.25050237
ATGGCCG15800.027.3268169
GGCACCC62600.027.25361635
AGTCCGC62950.027.1326947
CAGTCCG61350.026.825896
CGGCGTA5900.025.7847186
GTCCGCC66900.025.6340528
Produced by FastQC (version 0.11.5)