FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4004638
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG98710.24648919577749598No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC94060.23487765935397906No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC70880.17699477455889898No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT44310.11064670514538394No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC40870.1020566652965886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG21450.058.171484
TCTACGG21650.057.4744455
TACGGCC21650.056.6726467
CTACGGC23350.053.2900126
GCGCGGT49650.049.285269
TGGCGCG52000.047.3249915
GCCATAC25850.047.1978911
GGTCTAC23450.045.844913
ACGGCCA27500.045.1195568
CGGCCAT27900.043.853289
CGGCATA3250.043.7646521
GGGTCTA22850.043.421742
CGCGGTG56700.043.21923410
CCATACC28700.042.63195812
CATACCA28900.042.45652413
CCGAACT8600.041.7057367
GAACGCG29750.041.4025924
GGCCATA29800.041.17427410
ATCTCGT29350.041.1024335
CGGTTCA9300.040.511075
Produced by FastQC (version 0.11.5)