FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4021637
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG148030.36808394193707683No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG68060.16923456791351382No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC46090.11460507251151708No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG40560.10085445304984016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA52850.034.56580432
GCGGCAC54400.033.6543133
CGGCACC56800.032.3628734
TGGCCGT14650.032.35379470
CCCGTCG51650.030.44433844
TCGCCGG52900.029.71088448
CCGTCGC53400.029.59285445
GCACCCC63700.028.97416536
CCCCCGA64250.028.80184219
ACCCGCG64100.028.72630525
AGGACCG8200.027.67155870
CACCCCC67150.027.59714137
AGTCCGC68750.027.3102847
GGCACCC68550.027.27438735
CAGTCCG67950.026.8688416
ATGGCCG14250.025.96984369
GTCCGCC72400.025.8856938
GTTCCGT4150.025.8582571
TCCGCCC75200.025.6575669
CCCCGAC73150.025.20646320
Produced by FastQC (version 0.11.5)