FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4021637
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG101070.25131557124623627No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC100090.24887875260745812No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC77370.19238434498190662No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT46960.11676837069084056No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC41610.1034653301628168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG21750.059.927544
TCTACGG22250.058.2700845
TACGGCC22850.057.042637
CTACGGC23700.054.9967966
GCGCGGT52900.049.8016439
ACGGCCA27350.047.9100158
CCGAACT8550.047.2998567
TGGCGCG56300.046.9777315
GCCATAC27950.046.879211
GGGTCTA22800.046.7122232
CGGCCAT28250.046.5060589
GGTCTAC24750.045.1202743
CCATACC29950.044.21042612
GCCGGTT9300.043.8675583
GAACGCG29700.043.45475824
CGCGGTG60700.043.39993310
CATACCA30550.042.88947713
TGAACGC30250.042.7742623
ATCTCGT30200.042.71967335
CCCGATC30850.042.11846531
Produced by FastQC (version 0.11.5)