FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4133346
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG150440.36396662655388634No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG69020.16698335924454424No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC49610.12002382573343727No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG41940.10146743098690504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA50250.033.7971432
GCGGCAC53100.032.12267733
TGGCCGT14300.031.42146170
CGGCACC55500.030.8671434
TCGCCGG49500.030.6950748
CCCGTCG49600.030.37201144
CCGTCGC50600.029.85543845
CGTACGT2350.029.4326419
CCCCCGA60500.028.58128219
ACCCGCG62150.027.84946425
GCACCCC62600.027.49572436
ATGGCCG13900.026.61506769
CACCCCC65200.026.40664737
AGTCCGC66750.025.9996247
GGACGTA5250.025.83438143
GGCACCC66800.025.80980735
GTTCCGT4850.025.6903341
GACGTAT5150.025.67105144
CCCCGAC68650.025.29106120
CAGTCCG66150.025.0334266
Produced by FastQC (version 0.11.5)